Designed to maximize performance of LC systems, Shimadzu’s Shim-pack Velox columns with core shell technology enable you to achieve increased separations and faster analysis times on any LC platform. Whether developing a high efficiency LC separation method, transferring an existing method for increased throughput while maintaining resolution, or are trying to improve the resolution of a complex separation, Shim-pack Velox columns will satisfy your needs.
Column ruggedness is critical to any LC analysis and Shim-pack Velox core-shell columns deliver excellent column lifetime for even the most challenging sample matrices.

Shim-pack Velox column will deliver

• Increased resolution with maximum efficiency → improving separation and detection
• Faster separation without sacrificing performance → maximizing laboratory productivity and reducing cost of analysis
• Increased sample throughput → reducing overall analysis time
• Superior ruggedness → reducing cost of analysis
• Excellent reproducibility → maintaining analysis and data integrity

Selection Guide for Different LC Platforms
Column particle sizes and column volumes affect chromatography results significantly if the column configuration does not match　the LC system. As column particle size is reduced, or the column volume (ID and/or the length of the columns) decreases, the necessity for a lower dispersion system is increased.
Choosing the optimal column configuration for your LC system allows you to achieve improved chromatography.
The following table summarizes the starting recommendations of column configuration for each LC system.

LC System	HPLC		UHPLC-like	UHPLC
Particle size	2.7 μm & 5 μm		2.7 μm	1.8 μm & 2.7 μm
Column I.D.	4.6 mm (3.0 mm)		3.0 mm (2.1 mm)	2.1 mm
Column Length	100-250 mm		50-100 mm	≤150 mm

Column Chemistries
Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a wide variety of applications and challenging separations.

* For maximum lifetime, recommended maximum pressure for 1.8 μm particles is 80MPa.​

Lot to Lot Reproducibility
We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance.
From one lot to the next, every Shim-pack Velox column you purchase will perform consistently.

SP-C18
Designed and intended specifically for use under low pH condition, Shim-pack Velox SP (Sterically Protected)-C18 offers a well balanced retention profile with a long life time even under harsh, acidic condition needed for LC/MS(/MS) analysis.

• Sterically protected to resist strongly acidic (pH 1-3) mobile phase condition
• Well balanced retention profile
• Suitable for LC/MS(/MS) analysis

Low pH Stability
Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns maintain a stable retention profile under strongly acidic mobile phase condition (pH1) .

Simultaneous Analysis of 22 Preservative Agents
More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide range of commercial product within acceptable analytical times.

For Research Use Only. Not for use in diagnostic procedures.

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Shim-pack Velox SP-C18 Analytical Columns

C18
Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a wide range of applications such as pharmaceutical, food, environmental and clinical and neutrals at moderately low and mid-range pH.

• General purpose column for reversed-phase chromatography
• Highest hydrophobic retention among Shim-pack Velox series
• Compatible with moderately acidic to neutral mobile phases (pH 2-8)

Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment
The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 μm fully-porous ODS column described in the USP monograph is transferred to a new method with Shim-pack Velox C18 2.7 μm column, within USP allowable adjustments. Analytical time and solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

For Research Use Only. Not for use in diagnostic procedures.

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Biphenyl

Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. It is useful for fast separations in bioanalytical applications due to the increased retention of early eluting analytes such as dipolar, unsaturated and conjugated analytes.

• Complementary selectivity to alkyl phases
• Enhanced separation of aromatic compounds
• Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis

For Research Use Only. Not for use in diagnostic procedures.

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Shim-pack Velox Biphenyl Analytical Columns

Separating the Structural Isomers
Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two structural isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in chemical structures, could be relatively well resolved with shim-pack Velox Biphenyl column.

PFPP

Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is suitable for the analysis of halogenated compounds, positional isomers and charged bases.

• Alternative selectivity to C18 columns
• Suitable for positional isomers and halogenated compounds
• Offers increased retention for charged bases

Good Separation of Cis / Trans Stilbene
Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well separated with Shim-pack Velox PFPP column.

For Research Use Only. Not for use in diagnostic procedures.

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Shim-pack Velox PFPP Analytical Columns

HILIC

Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes.
Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for this application.

• Orthogonal selectivity to reversed phase chromatography
• Increased retention of polar analytes
• Increased MS sensitivity
• Direct compatibility with sample preparation eluates

Retention of Nucleosides
Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well retained and separated with Shim-pack Velox HILIC column.

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Ordering Information
Shim-pack Velox HILIC Analytical Columns