Shim-pack Series
- Описание
- Size Exclusion Columns
- Ion Chromatography Columns
Описание
Packed Columns for High-Performance Liquid Chromatograph
For Research Use Only. Not for use in diagnostic procedures.
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Size Exclusion Columns
Size Exclusion Columns
Shim-pack GPS Series
Shim-pack GPC series columns are used for the determination of tetrahydrofuran (800 Series), chloroform (800C Series), and dimethylformamide (800D series). The technique of GPC does not utilize such chemical reactions as partition, adsorption, and ion exchange, but a physical reaction consisting of a separation based on molecular size of the sample components. Therefore, this method is suitable for the measurement of molecular weight distribution of high polymers and oligomers. Shim-pack GPC series are packed with polystyrene polymers with respective degrees of cross-linking in order to meet exact analysis requirements, ranging from analysis of high polymers to that of oligomers. GPC-80M (80MC, 80MD) are mixed gel columns.
Analysis Examples
Calibration Curves
■Conditions
| Mobile Phase: | Tetrahydrofuran |
| Flow Rate: | 1.0 mL/min |
| Col. Temp.: | Ambient |
| Detection: | UV 254 nm |
Analysis of Polystyrene Standard
■Conditions
| Column: | Shim-pack GPC-80M (2 columns in series) (P/N: 228-20810-91) |
| Mobile Phase: | Tetrahydrofuran |
| Flow Rate: | 1.0 mL/min |
| Col. Temp.: | Ambient |
| Detection: | UV 254 nm |
Determination in Tetrahydrofuran
| Column | Exclusion Limit (polystyrene) |
Dimensions (Length x I.D., mm) |
P/N |
|---|---|---|---|
| Shim-pack GPC-801 | 1.5 x 103 | 300 x 8.0 | 228-20803-91 |
| Shim-pack GPC-802 | 5 x 103 | 300 x 8.0 | 228-20804-91 |
| Shim-pack GPC-8025 | 2 x 104 | 300 x 8.0 | 228-20805-91 |
| Shim-pack GPC-803 | 7 x 104 | 300 x 8.0 | 228-20806-91 |
| Shim-pack GPC-804 | 4 x 105 | 300 x 8.0 | 228-20807-91 |
| Shim-pack GPC-805 | 4 x 106 | 300 x 8.0 | 228-20808-91 |
| Shim-pack GPC-806 | 4 x 107 | 300 x 8.0 | 228-20809-91 |
| Shim-pack GPC-80M | 4 x 107，Mixed gel | 300 x 8.0 | 228-20810-91 |
| Shim-pack GPC-800P | Guard Column | 10 x 4.6 | 228-20812-91 |
Determination in Chloroform
| Column | Exclusion Limit (polystyrene) |
Dimensions (Length x I.D., mm) |
P/N |
|---|---|---|---|
| Shim-pack GPC-801C | 1.5 x 103 | 300 x 8.0 | 228-20803-92 |
| Shim-pack GPC-802C | 5 x 103 | 300 x 8.0 | 228-20804-92 |
| Shim-pack GPC-8025C | 2 x 104 | 300 x 8.0 | 228-20805-92 |
| Shim-pack GPC-803C | 7 x 104 | 300 x 8.0 | 228-20806-92 |
| Shim-pack GPC-804C | 4 x 105 | 300 x 8.0 | 228-20807-92 |
| Shim-pack GPC-805C | 4 x 106 | 300 x 8.0 | 228-20808-92 |
| Shim-pack GPC-806C | 4 x 107 | 300 x 8.0 | 228-20809-92 |
| Shim-pack GPC-80MC | 4 x 107，Mixed gel | 300 x 8.0 | 228-20810-92 |
| Shim-pack GPC-800CP | Guard Column | 10 x 4.6 | 228-20812-92 |
Determination in Dimethylformamide
| Column | Exclusion Limit (polystyrene) |
Dimensions (Length x I.D., mm) |
P/N |
|---|---|---|---|
| Shim-pack GPC-801D | 1.5 x 103 | 300 x 8.0 | 228-20803-93 |
| Shim-pack GPC-802D | 5 x 103 | 300 x 8.0 | 228-20804-93 |
| Shim-pack GPC-8025D | 2 x 104 | 300 x 8.0 | 228-20805-93 |
| Shim-pack GPC-803D | 7 x 104 | 300 x 8.0 | 228-20806-93 |
| Shim-pack GPC-804D | 4 x 105 | 300 x 8.0 | 228-20807-93 |
| Shim-pack GPC-805D | 4 x 106 | 300 x 8.0 | 228-20808-93 |
| Shim-pack GPC-806D | 4 x 107 | 300 x 8.0 | 228-20809-93 |
| Shim-pack GPC-80MD | 4 x 107，Mixed gel | 300 x 8.0 | 228-20810-93 |
| Shim-pack GPC-800DP | Guard Column | 10 x 4.6 | 228-20812-93 |
Shim-pack Diol Series
Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. GFC is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. Due to the hydrophilic hydroxyl group, Shim-pack Diol series can be used in high-speed GFC and provide sharp peaks during the analysis of protein and biochemicals (such as enzymes).
Analysis Examples
Calibration Curves
■Peaks
- Blue dextran 2000
- Thyroglobulin
- y-Globulin
- Transferrin
- Human serum albumin
- Ovalbumin
- β-Lactoglobulin
- Chymotrypsin
- Myoglobin
- Lysozyme
- Ribonuclease A
- Cytochrome C
- Gly-Tyr
■Conditions
| Column: | Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm) |
| Mobile Phase: | A) 10mM phosphate buffer solution (pH 7) B) 0.2M sodium sulfate |
| Flow Rate: | 1.0 mL/min |
| Col. Temp.: | Ambient |
| Detection: | UV 280 nm |
Analysis of Protein Standard
■Peaks
- Glutamate dehydrogenase
- Lactate dehydrogenase
- Enolase
- Adenylate kinase
- Cytochrome C
■Conditions
| Column: | Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm) (P/N: 228-14776-92) |
| Mobile Phase: | A) 10mM phosphate buffer solution (pH 7) B) 0.1M sodium chloride |
| Flow Rate: | 1.0 mL/min |
| Col. Temp.: | Ambient |
| Detection: | UV 280 nm |
Product Information
| Column | Stationary phase |
Particle Size (µm) |
Dimensions (I.D. x Length, mm) |
P/N |
|---|---|---|---|---|
| Shim-pack Diol-150 | Diol group | 5 | 7.9 x 250 | 228-14775-91 |
| 7.9 x 500 | 228-14775-92 | |||
| Shim-pack Diol-300 | Diol group | 5 | 7.9 x 250 | 228-14776-91 |
| 7.9 x 500 | 228-14776-92 | |||
| Pre-column Diol * | Diol group | 10 | 4.0 x 50 | 228-16367-91 |
* Installed between the liquid pump and the sample injector to protect Shim-pack Diol column.
For Research Use Only. Not for use in diagnostic procedures.
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Please contact us to check the availability of these products in your country.
Ion Chromatography Columns
Shim-pack IC Series
Ion chromatography (IC) is used for analysis of inorganic and organic ions. It is categorized as suppressor IC and non-suppressor IC. Non-suppressor IC is composed of a conventional HPLC system combined with a conductivity detector, while suppressor IC requires an extra suppressor.
| Non- Suppressor Type |
Anion Analysis |
Shim-pack IC-A1 |
Capable of analyzing inorganic anions. Compatible with acid or basic mobile phases of wide pH range. |
|---|---|---|---|
| Shim-pack IC-A3 |
Provides outstanding separation efficiency in the analysis of inorganic anions and acid ions, especially when weakly acid mobile phase is used. | ||
| Cation Analysis |
Shim-pack IC-C1 |
Capable of analyzing cations such as alkaline metal ions, alkali earth metal ions, transition metal ions, rare earth metal ions, and alkyl amines. | |
| Shim-pack IC-C4 |
Capable of simultaneous analysis of alkali metal ions and alkaline earth metal ions. Enhances the separation of sodium and ammonium ions. |
| Suppressor Type |
Anion Analysis |
Shim-pack IC-SA2 |
Capable of determining fluoride ions, chloride ions, nitrite ions, bromide ions, nitrate ions, phosphate ions and sulfuric acid ions. |
|---|---|---|---|
| Shim-pack IC-SA3 |
Provides high-resolution performance when analyzing fluoride ions, chloride ions, nitrous acid ions, bromide ions, nitric acid ions, phosphate ions and sulfuric acid ions as well as chlorous acid ions, chloric acid ions and bromic acid ions. | ||
| Cation Analysis |
Shim-pack IC-SC1 |
Also can be used in non-suppressor IC. Suitable for the analysis of water quality. |
Product Information
| Column | Stationary phase |
Particle Size (µm) |
Dimensions (I.D. x Length, mm) |
P/N | Guard Column |
|---|---|---|---|---|---|
| Shim-pack IC-A1 |
Quaternary ammonium group |
12.5 | 4.6 x 100 | 228-17733-91 | 228-17734-91 |
| Shim-pack IC-A3 |
Quaternary ammonium group |
5 | 4.6 x 150 | 228-31076-91 | 228-31076-92 |
| Shim-pack IC-A3 (S) *1 |
Quaternary ammonium group |
5 | 2.0 x 150 | 228-33366-91 | |
| Shim-pack IC-C1 *2 |
Sulfone group |
10 | 5.0 x 150 | 228-17737-91 | 228-17738-91 |
| Shim-pack IC-C1 PEEK |
Sulfone group |
10 | 4.6 x 100 | 228-33497-91 | 228-33497-92 |
| Shim-pack IC-C4 |
Carboxyl group |
7 | 4.6 x 150 | 228-41616-91 | 228-59900-91 (Cartridge + Holder) |
| 228-59900-92 (Cartridge only) |
|||||
| Shim-pack IC-SA2 |
Quaternary ammonium group |
/ | 4.0 x 250 | 228-38983-91 | 228-38983-92 |
| Shim-pack IC-SA3 |
Quaternary ammonium group |
/ | 4.0 x 250 | 228-41600-91 | 228-41600-92 |
| Shim-pack IC-SC1 |
Quaternary ammonium group |
6 | 4.6 x 150 | 228-36605-91 | 228-36605-92 |
*1 Shim-pack IC(S) series are for semi-micro LC. PIA-1000 is required.
*2 During the analysis of alkali metal ions with the Shim-pack IC-C1, it is recommended to use a pre-column Shim-pack IC-PC1 (P/N：228-17744-91) installed between the liquid pump and the sample injector.
Shim-pack IC Series
Shim-pack IC-C4 is a cation analysis column for non-suppressor IC. Because the pH of the mobile phase can be changed by selecting a different combination of acid and base in eluent, non-suppressor IC enables various kinds of analysis.
High Resolution of Na+ and NH4+
High resolution of Na+ and NH4+ has been achieved by improving the peak shape of Na+. The influence on the peak shape of NH4+ from a high concentration of Na+ has been reduced, making it possible to analyze tap water of normal concentration under standard mobile phase conditions. The resolution can be further improved by using a mobile phase treated with 18-crown-6 additive.
Analyses of a Standard Mixture of 5 Cations
Calibration Curves
| ■Conditions | ||
| Column | ： | Shim-pack IC-C4 (150 mmL × 4.6 mmI.D., 7 μm) (P/N: 228-41616-91) |
| Mobile Phase | ： | 2.5 mmol/L oxalic acid |
| Flow Rate | ： | 1.0 mL/min |
| Col. Temp. | ： | 40 °C |
| Detection | ： | CDD |
| Injection Vol. | ： | 50 µL |
Flexible Mobile Phase Selection
Due to the features of non-suppressor IC, flexible mobile phase composition can be used. Besides normal inorganic cations, Shim-pack IC-C4 is capable of analyzing transition metals by using a mixed mobile phase.
Analyses of a Standard Mixture of 5 Cations
| ■Conditions | ||
| Column | ： | Shim-pack IC-C4 (150 mmL × 4.6 mmI.D., 7 μm) (P/N: 228-41616-91) |
| Mobile Phase | ： | A) 2.5 mmol/L oxalic acid B) 2.5 mmol/L methanesulfonic acid A/B = 60/40 (v/v) |
| Flow Rate | ： | 1.0 mL/min |
| Col. Temp. | ： | 40 °C |
| Detection | ： | CDD |
| Injection Vol. | ： | 50 µL |
Analysis of Anions in Water
Shim-pack IC-SA2 was developed for anion analysis on Shimadzu Ion Chromatograph HIC-SP (suppressor type). It is capable of analyzing fluoride ions, chloride ions, nitrite ions, bromide ions, nitrate ions, phosphate ions, sulfate ions, etc., making it possible to be used in both tap water and environmental water analysis.
Analysis of Standard Anion Samples
Analysis of Tap Water
Calibration Curves
| ■Peaks | |
| 1. F | ：1mg/L |
| 2. Cl | ：1mg/L |
| 3. NO2 | ：1mg/L |
| 4. Br | ：1mg/L |
| 5. NO3 | ：1mg/L |
| 6. PO4 | ：1mg/L |
| 7. SO4 | ：1mg/L |
| ■Conditions | ||
| Column | ： | Shim-pack IC-SA2 (250 mmL x 4.0 mmI.D.) (P/N: 228-38983-91) |
| Mobile Phase | ： | 12 mmol/L sodium hydrogen carbonate 0.6 mmol/L sodium carbonate |
| Flow Rate | ： | 1.0 mL/min |
| Col. Temp. | ： | 30 °C |
| Detection | ： | CDD |
| Injection Vol. | ： | 50 µL |
Environmental water and mineral water usually contains silicate. In this kind of analysis, although the water sample contains a large amount of silicic acid, fluoride ions can still be separated and determined with high solution. (same mobile phase as above was used)
For Research Use Only. Not for use in diagnostic procedures.
This page may contain references to products that are not available in your country.
Please contact us to check the availability of these products in your country.
