Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.

Expanding the Possibilities of UHPLC

Superb Functionality
Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shimadzu addressed these features by incorporating superb technology as a fundamental element. Furthermore, improved hardware components support high-speed analyses, which have accelerated via advances in column technology.
As the most accessible UHPLC available, with a pressure resistance of 66 MPa, the Nexera XR truly provides the optimal solution for a diverse range of applications.

Extensive Lineup
A solvent delivery unit compatible with small-capacity, low-pressure gradient units is the latest addition to the Nexera XR lineup. With its modular design, the Nexera XR allows configuration of systems geared to meet specific application requirements. Each module delivers excellent, reliable performance across an extensive analytical range, from HPLC to UHPLC.

Balancing Performance with User-Friendliness
The most accessible UHPLC available, the Nexera XR provides excellent performance while ensuring ease-of-use. In addition, although the Nexera XR is in the UHPLC category, the replacement frequency for consumables has been kept to a minimum, making the ease of maintenance comparable to that of an HPLC system.

Method Compatibility
Constructed with a focus on compatibility with general UHPLC systems, the Nexera XR is optimized for method transfer from competing systems. In addition, the system capacity can be changed to suit the objective, enabling smooth HPLC method transfer.

Detectors Offering World-Leading* Sensitivity
The SPD-M30A photodiode array detector and the RF-20Axs fluorescence detector offer world-leading sensitivity. Equipped with a temperature control function to suppress the effects of room temperature fluctuations, these detectors achieve excellent baseline stability and reproducibility.

* As of August 2013, according to Shimadzu survey.

For Research Use Only. Not for use in diagnostic procedures.

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Жидкостные хроматографы SHIMADZU: объекты анализа, выполняемые ГОСТы

The Optimal Autosampler for MS Front End LC

Ultra High Performance Liquid Chromatograph
Low Carryover Supports MS with Higher Accuracy
Low carryover is essential for high-sensitivity LC/MS/MS systems. Shimadzu autosamplers have been engineered with this in mind. The structure of the needle seal and the materials for the parts in contact with fluids have been thoroughly revised to keep sample component adsorption to the absolute minimum.
As a result, no carryover has been detected even for substances prone to adsorption.

Improved Throughput
The SIL-20AXR /20ACXR autosamplers require a mere 10 seconds (under specified conditions) for sample injection. Combine with high-speed separation columns to achieve analysis cycles of one minute or less. Using a rack changer makes it possible to significantly increase the number of samples that can be loaded at one time. In addition, refrigerated storage is available, allowing processing of samples in sequence by simply placing them in the changer after completing preparation.

Excellent Reproducibility
The Nexera XR is equipped with a high-performance scale to accurately measure samples, achieving excellent reproducibility on the order of 0.2 % RSD or less, even for trace-quantity injections.

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Solvent Delivery Performance that Supports High-Accuracy Analysis

System Capacity with a Focus on Compatibility
Designed to be compatible with general UHPLC systems, Nexera XR can be controlled from competing workstations, and can use methods created with competing systems.

Baseline Stability
Baseline stability is affected by numerous factors, including the performance of the pump, oven, detector, and other components. By utilizing temperature-controlled detectors, the Nexera XR improves baseline stability for the system as a whole.

Supports a Variety of Analysis Conditions
With its 66 MPa pressure resistance, Nexera XR expands the scope of high-speed, high-resolution analysis. This may include analysis under room temperature conditions, improvement of separation using long columns, and the use of a water/methanol mobile phase.

Standard Nexera XR System
This system is equipped with a low-pressure gradient system recognized for its concentration accuracy.
This high-pressure mixed system provides excellent gradient response. It can be configured as a quaternary system by adding a low-pressure gradient unit.

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Detectors Featuring World-Leading Sensitivity

Photodiode Array Detectors
The lineup of photodiode array detectors consists of the SPD-M20A and SPD-M30A. Both models are temperature-controlled, heightening data reliability and baseline stability with respect to room temperature fluctuations. The SPD-M30A incorporates a new type of capillary cell to function as a higher-sensitivity and lower-dispersion system. The SPD-M20A is an easy-to-use model that covers a wide detection range.

85 mm Optical Path Length Significantly Enhances Sensitivity
The SPD-M30A high-sensitivity cell is also effective under HPLC conditions. The analysis below shows a comparison of measurements of caffeine in a commercial soft drink using the SPD-M20A and SPD-M30A. It is apparent that the SPD-M30A improves the S/N ratio by up to eleven times.

New Analytical Techniques to Better Utilize PDA Detector Data
This technique fully separates unseparated peaks and visualizes small peaks hidden by a principal component. It is convenient when poor separation occurs under conditions for accelerated analysis and for quantitation of small peaks overlapped by a principal component peak.

* i-PDeA: Intelligent Peak Deconvolution Analysis

This powerful technique permits quantitation of samples in the high-concentration range. It enables the simultaneous analysis of high- and ultra-low-concentration samples and the creation of calibration curves across a broad concentration range.

** i-DReC: Intelligent Dynamic Range Extension Calculator

Fluorescence Detectors
The RF-20A/20Axs fluorescence detectors offer world-leading* sensitivity and ease of maintenance. The high-sensitivity RF-20Axs model incorporates a temperature-controlled cell with cooling functionality. This maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity.

* As of August 2013, according to Shimadzu survey.

Ultra-High-Sensitivity Analysis of Anthracene (RF-20Axs)
An S/N ratio of 21.5 was achieved for an injection of 10.48 fg anthracene (RF-20Axs). This is equivalent to approx. 1.5 fg limit of detection (S/N ratio = 3), which is excellent.

Multi-Component, High-Sensitivity UFLC Analysis
The highly sensitive simultaneous analysis of multiple components requires detection at the optimal wavelengths. The RF-20A/20Axs permit ultra fast, high-sensitivity multi-component analysis using wavelength switching via a time program.

Support for Improved Quantitative Analysis Accuracy: Utility of Four-Wavelength Measurement Function
Using detection at a single wavelength when performing multicomponent simultaneous analysis of components with different optimal detection wavelengths necessitates sacrificing sensitivity for certain components. The RF-20A/20Axs detectors eliminate this issue by incorporating a four-wavelength measurement function that permits detection of each component at the optimal wavelength.
Detection using wavelength switching in the left-hand diagram exhibits incomplete separation in area (1) and one peak of reduced size in area (2). In such a case, setting up to four optimal wavelengths enhances the quantitative analysis accuracy by reducing the effects of adjacent peaks and improving sensitivity.

Analysis of Polycyclic Aromatics by Four-Wavelength Simultaneous Measurement (Elution sequence shown in previous diagram)

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For More Efficient Method Development

Compatible with the Method Scouting System, Which Automates the Method Development Process
The Method Scouting method development system acquires data in sequence by automatically switching between up to 96 combinations of unique separation conditions. Automated functionality enables continuous data acquisition overnight for more efficient method development.

Automation Reduces Analysis Preparation Time and Prevents Errors
The Method Scouting system not only significantly reduces the preparation time involved in analysis work, but can also prevent human errors in the configuration of method files and schedules. With up to 6 columns, 8 mobile phases, and 10 gradient settings, there are 96 conceivable combinations. This system streamlines the process for more efficient and accurate method development.

Improved Method Development Efficiency Via Utilization of Equipment Overnight
Once analysis starts, special software is used to automatically switch the columns and mobile phases to implement analysis. Analysis can be performed overnight and on holidays when users are not present, freeing them from the burden of creating method files, preparing mobile phases, and even switching columns. These synergistic effects can improve the throughput involved in method development more than tenfold.

Fast Data Browsing
The ability to quickly search the large quantity of data obtained from method scouting for suitable conditions is important. The data browser function provided with LabSolutions meets this need by displaying a list of these chromatograms and analysis results.
In addition to retention time, area, and height, analysis results include the degree of separation, separation coefficients, theoretical plate numbers, symmetry coefficients and other indices for chromatogram evaluation, supporting the process of checking the large amount of data for optimal conditions.

Quantitation of Scouting Results via CLASS-Agent Report
CLASS-Agent Report enables quantitation in order to search the data obtained from method scouting for optimal data. With CLASS-Agent Report, numerical data and graphs can be created, utilizing Microsoft Excel, from calculations based on the degree of separation and the number of peaks*. As a result, in addition to visual comparison of chromatograms, determinations can be made based on quantitation of determination standards.

* For evaluation methods using Agent Report, refer to the technical reports separately.

Nexera Method Scouting System
This comprehensive method development support system automatically switches between combinations of mobiles phases and columns.

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Simultaneous Control of HPLC and GC Systems
LabSolutions enables operation and control of LCs and GCs with the same interface. It allows simultaneous control of up to 4 systems from a single PC. Instrument information for up to 16 systems can be registered to a single PC, providing for a flexible laboratory environment that allows users to switch between networked LC and GC systems. Sharing the operating environment for LC and GC analysis reduces operator training time.

Comprehensive Quantitative Results Window
A quantitative results window enables easy review of data results and each chromatogram. LabSolutions supports the QC check by accuracy % and the range check to see whether a quantitative value falls within the range of the calibration curve; in addition, it supports calculation of impurity peak amounts, S/N ratio, and peak valley ratio. These various functions greatly reduce the time required for data analysis.

Customize the Operation Platform to Fit Your Workflow
LabSolutions allows users to customize the screen icons and layouts; therefore, the graphical user interface can be adapted according to individual preferences and workflow. Even the rounding method and number of displayed digits for the calculation results, such as area, height, concentration and column performance parameters, can be defined in the system for consistency and individual laboratory requirements.

Powerful Regulatory Compliance and Data Management for a Paperless Laboratory
System administration features, including system policy, user administration, log browser, and audit trail, are provided to assist users in FDA 21 CFR Part 11 compliance, which is supported and configurable on a stand-alone workstation as well as in a network environment. In addition, automatic registration of PDF files, which can be generated by LabSolutions as a standard function, into the database is available to support paperless operations in an analytical laboratory.

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XR-ODS II High-Separation Column

The Shim-pack XR-ODS, which was developed with the aim of increasing the speed attained under standard column pressure conditions, has been joined by the Shim-pack XR-ODS II, which was developed in order to facilitate high-speed, high-separation analysis for a wider range of applications, such as those involving methanol mobile phases, by using a new long column.
The Shim-pack XR-ODS II is a high-separation analysis column that is suited to use in combination with the Nexera XR and that can also be used for high-temperature analysis.
Reducing the particle size of the packing material to an optimum level and unifying the particle diameter help suppress flow resistance and ensure excellent stability. As with the Shim-pack XR-ODS, quality control is performed in accordance with the standards indicated in the quality assurance document provided with the column.

XR-ODS II Lineup

Particle diameter of packing material: 2.2 µm
Pore size: 8 nm
Pressure resistance: 60 MPa

Example of High-Separation Analysis
The results obtained by performing the high-separation analysis of polycyclic aromatics using the Nexera XR and the XR-ODS II are shown below. With general-purpose LC, the resolution, RS, for isomers of benzofluoranthene was 1.25, whereas with the Nexera XR, this was significantly increased to 2.12. With general-purpose LC, the analysis time was approximately 50 minutes, whereas with the Nexera XR, it was reduced to approximately half this. One advantage of increased speed is reduced consumption of mobile phase.

Analysis Conditions
Column: Shim-pack XR-ODS II (150 mm (L) x 3 mm (i.d.))
Column temperature: 40°C
Mobile phase: Water/acetonitrile = 35/65
Flow rate: 0.9 mL/min
Detection: 254 nm

The Nexera XR (pressure resistance: 66 MPa) also helps expand the range of analysis methods that can be used by, for example, allowing the use of water/methanol mobile phases and long columns, and enabling analysis to be performed at room temperature.
The results obtained by analyzing the catechins in green tea using a water/acetonitrile mobile phase and a water/methanol mobile phase are shown below. It can be seen that the separation selectivity, as represented by the elution order and the separation between components, differs between the methanol and acetonitrile systems. This example illustrates how the Nexera XR helps increase the range of available analysis methods, a feature that is useful for method development.

Analysis Conditions
Column: Shim-pack XR-ODS II (75 mm (L) x 3 mm (i.d.))
Column temperature: 40 °C
Mobile phases: Phosphate buffer solution / acetonitrile (top), phosphate buffer solution / methanol (bottom), gradient elution
Flow rate: 1.0 mL/min
Detection: 230 nm

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