LCMS-8045
Надёжный тройной квадрупольный масс-спектрометр для повседневного использования
- Описание
- Performance
- Software
- Upgrade
Описание
Тройной квадрупольный масс-спектрометр
Тройной квадрупольный масс-спектрометр LCMS-8045 занимает среднее положение в линейке жидкостных масс-спектрометров UFMS: он обладает такими же показателями быстродействия, как LCMS-8050/8060, а благодаря использованию источника ионизации электроспреем (ESI) с нагреваемым газом, его чувствительность существенно увеличена по сравнению с младшими моделями. Максимальная скорость сканирования составляет 30 000 а.е.м./с, время переключения полярности ионизации 5 мс.
Надежный прибор для повседневного использования
При разработке LCMS-8045 особое внимание уделено обеспечению долговременной стабильности работы прибора. Конструкция источника ионизации и ионной оптики оптимизирована таким образом, чтобы максимально снизить любые возможные загрязнения. Это позволяет проводить анализ сотен и тысяч образцов со сложной матрицей, таких как экстракты из пищевых продуктов и биологических жидкостей без снижения чувствительности и затрат на профилактическое обслуживание прибора.
ПО LabSolutions LCMS и пакеты аналитических методик
Для интенсификации работы аналитических лабораторий SHIMADZU комплектует свои тройные квадрупольные масс-спектрометры готовыми пакетами аналитических методик (Method Packages), которые представляют собой комплексное решение аналитической задачи, включая метод-файлы с условиями хроматографического разделения и работы масс-спектрометра в режимах MRM и MRM/Product ion Scan, калибровочные данные и библиотеки масс-спектров продуктов фрагментации.
► Жидкостные хроматомасс-спектрометры SHIMADZU: объекты анализа, выполняемые ГОСТы
Сборник примеров практического применения
Performance
High Performance for a Variety of Applications
Veterinary Drugs
Nitrofuran metabolites in fish
Nitrofurans are a class of synthetic antibiotics. The use of nitrofurans for edible animal and fishery products is banned in many countries. The chromatogram below shows the recovery of nitrofuran metabolites from fish using the LCMS-8045. Excellent linearity and accurate quantitative results ranging from 0.05 to 20 ng/mL were obtained. The LCMS-8045 is shown to be an excellent platform for routine high-sensitivity analysis in challenging matrices.
| Compound | Calibration range (ng/mL) |
Accuracy (%) |
LOD (ng/mL) |
|---|---|---|---|
| AMOZ | 0.05 — 20 | 94.5 — 103.8 | 0.00024 |
| SEM | 0.05 — 20 | 93.2 — 111.6 | 0.015 |
| AHD | 0.05 — 20 | 95.5 — 102.1 | 0.001 |
| AOZ | 0.05 — 20 | 94.0 — 108.4 | 0.00096 |
*Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.
Residual Pesticides
Simultaneous analysis of residual pesticides in herbal supplements
Regulatory agencies worldwide provide oversight for an extensive and ever-increasing list of pesticides in order to promote food safety and protect public health. The MRM chromatogram below shows the simultaneous analysis of 153 pesticides spiked into Codonopsis pilosula at 0.01 mg/kg. Plant matrices can be very challenging due to background interferences, but the LCMS-8045 provides enough sensitivity to analyze most pesticides at the trace level of 10 parts per billion. Many pesticides methods also benefit from polarity switching, which the LCMS-8045 can accomplish in just 5 msec.
Use Synchronized Survey Scan (SSS) to obtain both qualitative and quantitative information from a single injection. Full scan MS2 spectra will be obtained when an MRM peak intensity threshold is exceeded.
Water Quality Analysis
High-sensitivity analysis of 21 pharmaceuticals and personal care products in less than eight minutes
Pharmaceutical and personal care products (PPCPs) encompass a family of compounds used by individuals for health and cosmetic purposes. As populations increase, and the use of these products increases, PPCPs will become a significant threat to our water supply. The LCMS-8045 is capable of trace-level quantitation of many PPCPs without extensive sample preparation.
| No. | Compound | LOD (ng/mL) |
LOQ (ng/mL) |
No. | Compound | LOD (ng/mL) |
LOQ (ng/mL) |
|---|---|---|---|---|---|---|---|
| 1 | Lincomycin | 0.01 | 0.04 | 12 | Ifenprodil | 0.0075 | 0.022 |
| 2 | Trimethoprim | 0.01 | 0.04 | 13 | Erythromycin | 0.015 | 0.045 |
| 3 | Pirenzepine | 0.01 | 0.03 | 14 | Azithromycin | 0.01 | 0.03 |
| 4 | Ofloxacin | 0.01 | 0.03 | 15 | Clarithromycin | 0.0034 | 0.01 |
| 5 | Ciprofloxacin | 0.002 | 0.006 | 16 | Roxithromycin | 0.0071 | 0.022 |
| 6 | Sulphapyridine | 0.002 | 0.006 | 17 | Carbamazepine | 0.01 | 0.03 |
| 7 | Carbazochrome | 0.015 | 0.045 | 18 | DEET | 0.0062 | 0.018 |
| 8 | Metoprolol | 0.013 | 0.04 | 19 | Crotamiton | 0.005 | 0.015 |
| 9 | Dicorantil | 0.005 | 0.015 | 20 | Bezafibrate | 0.0025 | 0.075 |
| 10 | Sulfamethoxazole | 0.003 | 0.01 | 21 | Triclocarban | 0.01 | 0.03 |
| 11 | Antipyrine | 0.01 | 0.03 |
*Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.
Limit of quantitation (LOQ) was calculated at S/N = 10.
Bioanalysis
High-sensitivity quantitation of Amlodipine in plasma
Amlodipine is widely utilized for the treatment of high blood pressure and angina. A high-sensitivity method for the quantitation of amlodipine in human plasma using LCMS-8045 has been developed. Appropriate linearity ranging from 0.03 to 300 ng/mL with excellent accuracy was achieved. Both interday and intraday variabilities were assessed for quality control samples. Percent RSD (Relative Standard Deviation) results were less than 5%.
| Lv | Actual conc. (ng/mL) |
Calculated Conc. (ng/mL) |
Accuracy (%) |
|---|---|---|---|
| 1 | 0.003 | 0.003 | 104.5 |
| 2 | 0.01 | 0.01 | 97.2 |
| 3 | 0.03 | 0.031 | 103 |
| 4 | 0.1 | 0.107 | 107.1 |
| 5 | 0.3 | 0.268 | 89.4 |
| 6 | 1 | 1.062 | 106.2 |
| 7 | 3 | 2.958 | 98.6 |
| 8 | 10 | 10.293 | 102.9 |
| 9 | 30 | 27.37 | 91.2 |
| 10 | 100 | 98.722 | 98.7 |
| 11 | 300 | 303.619 | 101.2 |
| QC sample | Concentration (ng/mL) |
%RSD | accuracy (%) |
|
|---|---|---|---|---|
| Interday Variation (n=6) |
Low | 0.1 | 3.78 | 97.8-109.1 |
| Middle | 5 | 3.16 | 90.0-95.4 | |
| High | 240 | 0.85 | 95.9-97.6 | |
| Intraday Variation (n=18) |
Low | 0.1 | 4.5 | 87.5-110.3 |
| Middle | 5 | 4.92 | 88.1-102.9 | |
| High | 240 | 4.25 | 88.3-104.0 | |
For Research Use Only. Not for use in diagnostic procedures.
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Consistent High-Sensitivity Performance
Robust Ion Source and Sample Introduction System
Shimadzu’s MS interface includes a high-sensitivity heated ESI source, heated capillary sample introduction, and proprietary ion transfer optics. These design features combine to produce robust MS performance. 12,000 plasma samples were injected over 25 days (over 480 samples per day). Picogram levels of alprazolam were analyzed with an RSD of 3.42%.
Instrument Detection Limits: IDL
IDL is one measure of sensitivity for analytical instrumentation. The chromatograms below show the IDL for Reserpine and Chloramphenicol, obtained using ESI+ and ESI-, respectively. The IDL for Reserpine is 2.03 femtograms and 2.09 femtograms for Chloramphenicol.
Newly Designed Ionization Unit
Designed without cables or tubes, removing the new ionization unit is simple: release a one-touch lever to open the unit and lift it out. In addition, no tools are needed to detach the needles fitted in APCI and DUIS units, allowing for easy maintenance.
Easy System Maintenance Reduces Downtime
As with Shimadzu’s other triple quad systems, maintaining the LCMS-8045 is simple. Replacing the desolvation line (DL) and ESI capillary is quick and easy.
Additionally, the design allows users to replace the DL without breaking vacuum, providing greater uptime and usability.
For Research Use Only. Not for use in diagnostic procedures.
This page may contain references to products that are not available in your country.
Please contact us to check the availability of these products in your country.
Software
Optional Software Programs
LC/MS/MS Method Packages and MRM Libraries
A variety of method packages and MRM libraries, which include analysis conditions such as MRM parameters, enable efficient implementation of simultaneous multi-component analyses. The method parameter list included in these packages can be used to create methods that analyze targeted components only. These packages can save laboratories a great deal of method development time.
| Description | |
|---|---|
| Method package | Residual Pesticides |
| Veterinary Drugs | |
| Water Quality Analysis | |
| Drugs of Abuse | |
| Rapid Toxicology Screening | |
| Primary Metabolites | |
| Lipid Mediators | |
| Cell Culture Profilling | |
| MRM Library | Metabolic Enzymes in Yeast |
Traverse MS
Nitrofuran metabolites in fish
Multivariate Analysis Software Supports MRM Data
Traverse MS data analysis software is intended for high-speed processing of MRM data acquired with Shimadzu triple quadrupole LCMS systems in the field of targeted metabolomics. Using multiple samples and multiple components, the software is able to create graphical and statistical analysis for metabolic pathway analysis.
For Research Use Only. Not for use in diagnostic procedures.
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Please contact us to check the availability of these products in your country.
Upgrade
Upgrade to LCMS-8060
Enhanced performance for the LCMS-8045/8050
The world’s greatest sensitivity and ultra-fast technologies found in the LCMS-8060 are now available for the LCMS-8045/8050.
The LCMS-8050 Upgrade Kit includes three key components.
Upon installation, the LCMS-8045/8050 will deliver LCMS-8060 performance.
(UFsweeper III Collision Cell is additionally included in the LCMS-8045 Upgrade Kit.)
- Improved ion sampling efficiency DL (Desolvation Line)
- Improved ion guide with enhanced focusing capabilities UF-Qarray
- Enhanced vacuum efficiency Rotary pump
- Improved the CID efficiency UFsweeper III Collision Cell
Development of UF-Qarray Ion Guide using Shimadzu’s Simulation Technologies
The new UF-Qarray was designed to maximize sensitivity without compromising robustness or changing the minimal noise performance established in our previously patented Qarray. To optimize the design of the UF-Qarray, a number of simulation studies were carefully conducted using two platforms to model ion behavior under increased gas flow in the low vacuum region. FLUENT software was used to determine which pole shape would maximize ion focusing under gas flows generated by the enhanced pumping configuration (Fig. 1).
The second technology, newly developed Optdesign modelling software, was used to analyze the complex behavior of ion trajectories when voltages are applied to the expanded ion cloud (Fig. 2).
Using FLUENT and Optdesign simulation models, several prototypes were developed for testing, which led to the UF-Qarray pole geometry final design. This design ejects neutral gas particles in order to reduce noise while achieving greater ion focusing than the previous Qarray design.
Groundbreaking Technology for High Sensitivity
With the newly designed UF-Qarray, improved ion focusing efficiency results in greater signal intensity and a reduction in noise. By improving the ion sampling device (desolvation line) and the ion guide (UF-Qarray), and enhancing the vacuum efficiency, the LCMS-8060 defines sensitivity. These new technologies allow for improvements in sensitivity and robustness in all acquisition modes.
For Research Use Only. Not for use in diagnostic procedures.
This page may contain references to products that are not available in your country.
Please contact us to check the availability of these products in your country.
